Enzyme

Supplied
NEBuffer

% Activity in NEBuffers

1

2

3

4

Aat II

4

0

50

50

100

Acc I

4

50

50

10

100

Acc65 I

3 BSA

10

75

100

25

Aci I

3

25

50

100

50

Acl I

4 BSA

10

10

0

100

Afe I *

SE-Y

25

50

25

100

Afl II

2 BSA

50

100

25

100

Afl III

3 BSA

25

75

100

50

Age I

1

100

50

10

75

Ahd I

4 BSA

25

75

0

100

Ale I

4

10

20

10

100

Alu I

2

100

100

75

100

Alw I

4

50

100

10

100

AlwN I

4

50

100

50

100

Apa I @25°C

4 BSA

25

50

0

100

ApaL I

4 BSA

100

100

10

100

Apo I @50°C

3 BSA

10

75

100

75

Asc I

4

0

10

10

100

Ase I

3

NR

75

^dd100

NR

AsiS I

3 BSA

50

100

100

50

Ava I

4

10

75

10

100

Ava II

4

50

75

10

100

Avr II

2

100

100

50

100

Bae I @25°C

2 BSA SAM

50

100

50

75

BamH I

U^dd BSA

75

100

50

75

Ban I

4

50

100

50

100

Ban II

4

100

10

50

100

Bbs I

2

100

100

25

75

Bbv I

2

100

100

25

75

BbvC I

4

50

100

10

100

BceA I

U^dd BSA

25

NR

NR

75

Bcg I

U SAM

50

75

100

50

BciV I

4

100

25

10

100

Bcl I @50°C

3

50

100

^dd100

75

Bfa I

4

75

50

10

100

BfrB I

4 BSA

10

25

10

100

BfuA I @50°C

3

0

75

100

10

BfuC I

4 BSA

100

50

25

100

Bgl I

3

50

75

100

50

Bgl II

3

10

75

100

10

Blp I

4

50

100

10

100

Bme1580 I

2

75

100

75

75

BmgB I

3 BSA

0

25

100

10

Bmr I

2

75

100

75

100

Bmt I *

SE-W

25

100

10

50

Bpm I

3 BSA

75

100

100

100

Bpu10 I*

SE-K

0

25

100

25

BpuE I @25°C

2 SAM

75

100

10

75

Bsa I @50°C

3

75

75

100

100

BsaA I

3

75

75

100

75

BsaB I @60°C

2

75

100

100

100

BsaH I

4 BSA

50

100

100

100

BsaJ I @60°C

2

100

100

100

100

BsaW I @60°C

2 BSA

50

100

50

50

BsaX I

4

75

100

10

100

BseR I

2

100

100

75

100

BseY I

3

10

50

100

50

Bsg I

4 SAM

50

75

50

100

BsiE I @60°C

2 BSA

50

100

10

100

BsiHKA I @65°C

3 BSA

50

100

100

100

BsiW I @55°C

3

100

100

100

25

Bsl I @55°C

3

10

50

100

75

Bsm I @65°C

2

75

100

75

75

BsmA I @55°C

3

50

100

100

50

BsmB I @55°C

3

75

100

100

100

BsmF I @65°C

4 BSA

25

50

50

100

BsoB I @65°C

2

10

100

100

50

Bsp1286 I

4 BSA

25

25

25

100

BspCN I @25°C

1 BSA SAM

100

75

10

75

BspD I

4

25

75

50

100

BspE I

3

0

10

100

0

BspH I

4

10

100

50

100

BspM I

3

NR

NR

100

NR

Bsr I @65°C

3

0

50

100

10

BsrB I

2

50

100

100

100

BsrD I @60°C

2 BSA

50

100

50

75

BsrF I

U

25

100

75

75

BsrG I

2 BSA

25

100

10

100

BssH II @50°C

3

100

100

^dd100

100

BssK I @60°C

3 BSA

0

50

100

50

BssS I

3

0

50

^dd100

10

BstAP I * @60°C

SE-W

25

25

100

25

BstB I @65°C

4

75

50

25

100

BstE II @60°C

3

50

75

100

75

BstF5 I * @65°C

SE-Y

75

50

25

100

BstN I @60°C

2 BSA

10

100

100

75

BstU I @60°C

2

100

100

50

100

BstX I @55°C

3

25

100

100

50

BstY I @60°C

2

50

100

75

100

BstZ17 I

3

NR

NR

100

NR

Bsu36 I

3 BSA

0

25

100

0

Btg I

3 BSA

25

50

100

100

Bts II

4 BSA

100

50

50

100

Cac8 I

3

50

75

100

75

Cla I

4 BSA

10

50

50

100

Dde I

3

75

100

100

75

Dpn I

4

100

100

75

100

Dpn II

U

NR

NR

^dd100

NR

Dra I

4

75

75

50

100

Dra III

3 BSA

100

75

100

25

Drd I

4

25

50

10

100

Eae I

1

100

100

50

100

Eag I

3

10

25

100

10

Ear I

1

100

100

50

100

Eci I

2 BSA

50

100

100

50

EcoN I

4

100

100

75

100

EcoO109 I

4 BSA

100

100

75

100

EcoR I

U^dd

100

100

100

100

EcoR V

3 BSA

50

75

100

50

Fat I *

SE-G

10

100

50

50

Fau I * @55°C

SE-B

100

25

10

50

Fnu4H I

4

10

25

25

100

Fok I

4

50

100

75

100

Fse I

4 BSA

100

75

0

100

Fsp I

4

10

75

10

100

Hae II

4 BSA

75

100

50

100

Hae III

2

50

100

25

75

Hga I

1

100

75

50

100

Hha I

4 BSA

75

100

100

100

Hinc II

3 BSA

75

100

100

100

Hind III

2

50

100

10

50

Hinf I

2

75

100

75

75

HinP1 I

2

100

100

100

100

Hpa I

4

25

50

10

100

Hpa II

1

100

50

10

100

Hph I

4

NR

75

0

100

Hpy99 I

4 BSA

10

10

0

100

Hpy188 I

4

50

25

10

100

Hpy188 III

4 BSA

100

100

10

100

HpyCH4III

4

100

50

25

100

HpyCH4IV

1

100

25

10

25

HpyCH4V

4

50

50

25

100

I-Ceu I

4 BSA

10

10

0

100

I-Sce I

U BSA

10

50

50

50

Kas I

2 BSA

75

100

75

75

Kpn I

1 BSA

100

75

0

50

Mbo I

3

75

100

100

100

Mbo II

2

100

100

50

100

McrBC

2 BSA GTP

50

100

10

75

Mfe I

4

75

50

10

100

Mlu I

3

25

75

100

50

Mly I

4 BSA

50

50

25

100

Mme I

4 SAM

NR

NR

NR

100

Mnl I

2 BSA

75

100

50

75

Msc I

4

75

75

75

100

Mse I

2 BSA

75

100

75

75

Msl I

2

100

100

25

100

Msp I

2

75

100

50

50

MspA1 I

4 BSA

50

100

50

100

Mwo I @60°C

U

10

75

100

75

Nae I

1

100

75

10

75

N.Alw I

2

25

100

25

50

Nar I

1

100

75

75

75

N.BbvC IA

2

50

100

10

100

N.BbvC IB

2

50

100

10

100

N.BstNB I @55°C

U

0

10

10

10

Nci I

4

100

25

10

100

Nco I

4

100

100

100

100

Nde I

4

75

100

75

100

NgoM IV

4

100

50

10

100

Nhe I

2 BSA

100

100

10

100

Nla III

4 BSA

25

25

25

100

Nla IV

4 BSA

10

10

10

100

Not I

3 BSA

0

75

100

50

Nru I

U

0

10

75

10

Nsi I

U

25

25

50

10

Nsp I

2 BSA

100

100

0

100

Pac I

1 BSA

100

75

10

100

PaeR7 I

4

25

100

10

100

Pci I *

SE-O

25

75

100

50

PflF I

4 BSA

0

25

25

100

PflM I

3 BSA

0

100

100

50

PI-Psp I @65°C

U BSA

0

10

10

10

PI-Sce I

U BSA

0

0

0

0

Ple I

4

100

100

75

100

Pme I

4 BSA

0

50

10

100

Pml I

1 BSA

100

75

0

75

PpuM I

4

0

25

0

100

PshA I

4 BSA

50

75

10

100

Psi I *

SE-B

50

100

0

25

PspG I @75°C

2

75

100

50

100

PspOM I *

SE-Y

100

50

100

Pst I

3 BSA

75

75

100

50

Pvu I

3 BSA

10

75

100

10

Pvu II

2

100

100

100

100

Rsa I

1

100

100

50

100

Rsr II

4

25

75

10

100

Sac I

1 BSA

100

50

10

100

Sac II

4

25

75

10

100

Sal I

U BSA

0

0

50

0

Sap I

4

75

50

0

100

Sau3A I

U BSA

100

50

10

100

Sau96 I

4

50

100

100

100

Sbf I *

SE-Y

75

50

0

100

Sca I

U

25

75

^dd50

25

ScrF I

4

100

100

100

100

SexA I

3 BSA

25

25

100

100

SfaN I

3

0

75

100

25

Sfc I

4 BSA

75

50

10

100

Sfi I @50°C

2 BSA

0

100

10

100

Sfo I

2

25

100

50

50

SgrA I

4

100

50

10

100

Sma I @25°C

4

0

0

0

100

Sml I @55°C

4 BSA

25

75

25

100

SnaB I

4 BSA

25

50

25

^dd100

Spe I

2 BSA

75

100

25

75

Sph I

2

100

100

50

100

Ssp I

U

50

100

50

50

Stu I

2

100

100

50

100

Sty I

3 BSA

25

75

100

10

StyD4 I

2

10

100

100

100

Swa I @25°C

3 BSA

10

10

100

10

Taq I @65°C

U BSA

50

75

75

75

Tfi I @65°C

3

100

100

^dd100

100

Tli I @75°C

3 BSA

25

25

100

10

Tse I @65°C

3

75

100

100

75

Tsp45 I @65°C

1 BSA

100

25

0

75

Tsp509 I @65°C

1

100

100

100

NR

TspR I @65°C

4 BSA

25

50

25

100

Tth111 I @65°C

1

100

25

25

100

Xba I

2 BSA

0

100

75

75

Xcm I

2

10

100

50